Cytokeratin 19 antibody (C-Term)

Catalog No. ABIN462603

The Rabbit Polyclonal anti-Cytokeratin 19 antibody (Clone E16-L) (ABIN462603) specifically detects Cytokeratin 19 in IHC, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Cytokeratin 19 antibody (C-Term) (ABIN462603)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: E16-L

Product Details anti-Cytokeratin 19 Antibody

Binding Specificity: C-Term

Specificity: Human Cytokeratin 19

Purification: Purified

Immunogen: Peptide derived from C-terminal region of human cytokeratin 19.

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:25 - 1:100)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3920 was determined to be 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 20 mM Tris-HCl,  pH 8.0, 20 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: KRT19 / CK19 / Cytokeratin 19

Background: Name/Gene ID: KRT19

Synonyms: KRT19, CK19, Cytokeratin 19, Cytokeratin-19, Keratin 19, Keratin-19, CK-19, K19, K1CS, Keratin, type I, 40-kd

Gene ID: 3880

UniProt: P08727