TWEAK antibody (AA 35-84)

Catalog No. ABIN6740497

The Rabbit Polyclonal anti-TWEAK antibody is suitable to detect TWEAK in samples from Human, Mouse and Horse. It has been validated for WB.

Quick Overview for TWEAK antibody (AA 35-84) (ABIN6740497)

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Reactivity: Human, Mouse, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWEAK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TWEAK Antibody

Binding Specificity: AA 35-84

Specificity: Human TNFSF12 / TWEAK

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse (92%) Mouse (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human TNFSF12 (O43508, NP_003800). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%), Monkey, Marmoset (92%), Mouse (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TWEAK

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Alternative Name: TNFSF12 / TWEAK

Background: Name/Gene ID: TNFSF12
Family: TNF

Synonyms: TNFSF12, APO3/DR3 ligand, APO3L, DR3LG, APO3 ligand, TWEAK

Gene ID: 8742

NCBI Accession: NP_003800

UniProt: O43508

Pathways: Apoptosis