MARCH7 antibody (AA 288-337)

Catalog No. ABIN6740813

The Rabbit Polyclonal anti-MARCH7 antibody is suitable to detect MARCH7 in samples from Human, Mouse, Rat, Horse, Rabbit, Guinea Pig, Monkey, Chicken, Hamster and Xenopus laevis. It has been validated for WB.

Quick Overview for MARCH7 antibody (AA 288-337) (ABIN6740813)

Target: MARCH7 (Membrane-Associated Ring Finger (C3HC4) 7, E3 Ubiquitin Protein Ligase (MARCH7))

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Guinea Pig, Monkey, Chicken, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MARCH7 Antibody

Binding Specificity: AA 288-337

Specificity: Human MARCH7 / Axotrophin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Monkey, Mouse, Rat, Chicken, Xenopus (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa288-337 of human MARCH7 (Q9H992, NP_073737). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Rabbit, Horse, Opossum, Guinea pig, Turkey, Chicken, Xenopus (100%), Bovine, Bat, Zebra finch, Platypus (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MARCH7

Target: MARCH7 (Membrane-Associated Ring Finger (C3HC4) 7, E3 Ubiquitin Protein Ligase (MARCH7))

Alternative Name: MARCH7 / Axotrophin

Background: Name/Gene ID: MARCH7

Synonyms: MARCH7, AXO, Axotrophin, RNF177, AXOT, MARCH-VII, RING finger protein 177

Gene ID: 64844

NCBI Accession: NP_073737

UniProt: Q9H992