MARCH7 antibody (Internal Region)

Catalog No. ABIN238658

The Goat Polyclonal anti-MARCH7 antibody is suitable to detect MARCH7 in samples from Human. It has been validated for ELISA and IHC.

Quick Overview for MARCH7 antibody (Internal Region) (ABIN238658)

Target: MARCH7 (Membrane-Associated Ring Finger (C3HC4) 7, E3 Ubiquitin Protein Ligase (MARCH7))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MARCH7 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MARCH7 Antibody

Binding Specificity: Internal Region

Purpose: MARCH-VII / Axotrophin

Sequence: NFNQESEGRN T

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NFNQESEGRNT, from the internal region of the protein sequence according to NP_073737.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 125 kDa band in Human Spleen and Tonsil lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated si

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MARCH7

Target: MARCH7 (Membrane-Associated Ring Finger (C3HC4) 7, E3 Ubiquitin Protein Ligase (MARCH7))

Alternative Name: MARCH7

Background: MARCH7, membrane-associated ring finger (C3HC4) 7, AXO, AXOT, DKFZP586F1122, MARCH-VII, RNF177, axotrophin, membrane-associated RING-CH protein VII

Gene ID: 64844, 57438, 311059

NCBI Accession: NP_073737