MIA antibody

Catalog No. ABIN464852

This Rabbit Polyclonal antibody specifically detects MIA in ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for MIA antibody (ABIN464852)

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIA antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-MIA Antibody

Specificity: Human MIA

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIA.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 1 μg/mL-2 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a  pH 6 Sodium Citrate buffer or enzyme induced antigen retrieval with proteinase K at room temperature. Optimal concentrations and conditions may vary. ELISA: To detect hMIA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIA (LS-C104284) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIA. Western Blot: To detect hMIA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Alternative Name: MIA

Background: Name/Gene ID: MIA
Family: MIA

Synonyms: MIA, CD-RAP, Melanoma inhibitory activity

Gene ID: 8190

UniProt: Q16674