IL-31 antibody (Biotin)

Catalog No. ABIN464885

This Rabbit Polyclonal antibody specifically detects IL-31 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for IL-31 antibody (Biotin) (ABIN464885)

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-31 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL-31 Antibody (Biotin)

Specificity: Human IL-31

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-31.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hIL-31 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-31. To detect hIL-31 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-31 (LS-B7477) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-31. Western Blot: To detect hIL-31 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-31 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-31

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Alternative Name: IL31

Background: Name/Gene ID: IL31
Family: Interleukin

Synonyms: IL31, IL-31, Interleukin 31, Interleukin-31

Gene ID: 386653

UniProt: Q6EBC2