IL-15 antibody

Catalog No. ABIN464931

The Rabbit Polyclonal anti-IL-15 antibody is suitable to detect IL-15 in samples from Human. It has been validated for WB, ELISA and Neut.

Quick Overview for IL-15 antibody (ABIN464931)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-15 Antibody

Specificity: Human IL-15

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-15.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.15 - 0.2 μg/mL), SwELISA, WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/mL-1 mg/mL with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-15 (1 ng/mL), a concentration of 0.15-0.20 μg/mL of this antibody is required. ELISA: To detect hIL-15 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-15 (LS-C104326) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-15. Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-15

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Alternative Name: IL15

Background: Name/Gene ID: IL15
Family: Hormone

Synonyms: IL15, Interleukin-15, IL-15, Interleukin 15

Gene ID: 3600

UniProt: P40933

Pathways: JAK-STAT Signaling, Glycosaminoglycan Metabolic Process