CXCL12 antibody

Catalog No. ABIN465044

The Goat Polyclonal anti-CXCL12 antibody is suitable to detect CXCL12 in samples from Mouse. It has been validated for WB and ELISA.

Quick Overview for CXCL12 antibody (ABIN465044)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CXCL12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CXCL12 Antibody

Specificity: Mouse SDF-1alpha

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine SDF-1alpha.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Murine SDF-1alpha (100 ng/mL), a concentration of 2.5-3.5 μg/mL of this antibody is required. ELISA: To detect Murine SDF-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine SDF-1alpha (LS-C104407) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine SDF-1alpha. Western Blot: To detect Murine SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine SDF-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: SDF-1 / CXCL12

Background: Name/Gene ID: CXCL12
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL12, C-X-C motif chemokine 12, HIRH, IRH, SDF-1, SDF-1b, SDF1A, SDF1B, TPAR1, TLSF-a, TLSF-b, SDF-1a, SDF1, Sdf1alpha, Stromal cell-derived factor 1, TLSF, HSDF-1, PBSF, SCYB12

Gene ID: 6387

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling