TNFRSF11A antibody

Catalog No. ABIN465071

This Rabbit Polyclonal antibody specifically detects TNFRSF11A in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for TNFRSF11A antibody (ABIN465071)

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF11A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TNFRSF11A Antibody

Specificity: Human sRANK Receptor

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human sRANK Receptor.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 1 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hsRANK Receptor by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sRANK Receptor (LS-C104414) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANK Receptor. Western Blot: To detect hsRANK Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANK Receptor is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TNFRSF11A

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Alternative Name: TNFRSF11A / RANK

Background: Name/Gene ID: TNFRSF11A
Family: TNF Receptor

Synonyms: TNFRSF11A, CD265, CD265 antigen, ODFR, LOH18CR1, OPTB7, OSTS, PDB2, RANK, Receptor activator of NF-KB, TRANCER, FEO, OFE

Gene ID: 8792

UniProt: Q9Y6Q6

Pathways: NF-kappaB Signaling