TRAIL antibody

Catalog No. ABIN465135

The Mouse Monoclonal anti-TRAIL antibody (ABIN465135) specifically detects TRAIL in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for TRAIL antibody (ABIN465135)

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRAIL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TRAIL Antibody

Specificity: Human TRAIL/Apo2L

Purification: Protein A purified

Immunogen: E.coli derived recombinant Human TRAIL/Apo2L.

Type of Immunogen: Cell extract

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 4 μg/mL), WB (0.25 - 0.5 μg/mL)

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TRAIL/Apo2L (10 ng/mL), a concentration of 0.30-0.60 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human sTRAIL/APO2L when used with biotinylated antigen affinity purified anti-human sTRAIL/APO2L (LS-C104497) as the detection antibody at a concentration of approximately 1-2 μg/mL. Western Blot: To detect Human sTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sTRAIL/Apo2L is 0.25-1 ng/lane, under reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in Sterile water to a concentration of 1 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Alternative Name: TNFSF10 / TRAIL

Background: Name/Gene ID: TNFSF10
Family: TNF

Synonyms: TNFSF10, Apo-2L, APO2L, Apo-2 ligand, CD253, CD253 antigen, TRAIL, Protein TRAIL, TL2

Gene ID: 8743

UniProt: P50591

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity