CXCL9 antibody

Catalog No. ABIN465139

This Mouse Monoclonal antibody specifically detects CXCL9 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for CXCL9 antibody (ABIN465139)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CXCL9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL9 Antibody

Specificity: Human MIG

Purification: Protein A purified

Immunogen: E.coli derived recombinant Human MIG.

Type of Immunogen: Cell extract

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 4 μg/mL), Neut (5 - 10 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human MIG (100 ng/mL), a concentration of 5-10 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human MIG when used with biotinylated antigen affinity purified anti-human MIG (LS-C104825) as the detection antibody at a concentration of approximately 1-2 μg/mL. Western Blot: To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.50-2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 0.25-0.50 ng/lane, under non-reducing conditions and 2-4 ng/lane, under reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 500 μL sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Alternative Name: MIG / CXCL9

Background: Name/Gene ID: CXCL9
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL9, Crg-10, Humig, SCYB9, Small-inducible cytokine B9, C-X-C motif chemokine 9, CMK, MIG

Gene ID: 4283

UniProt: Q07325