IL-7 antibody

Catalog No. ABIN465146

The Mouse Monoclonal anti-IL-7 antibody is suitable to detect IL-7 in samples from Human. It has been validated for WB, ELISA and Neut.

Quick Overview for IL-7 antibody (ABIN465146)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-7 Antibody

Specificity: Human IL-7

Purification: Protein A purified

Immunogen: E. coli derived recombinant Human IL-7.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 4 μg/mL), Neut (0.1 μg/mL), WB (1 - 2 μg/mL)

Usage: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-7 when used with biotinylated antigen affinity purified anti-human IL-7 (LS-C104559) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1-2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2-4 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL7

Background: Name/Gene ID: IL7
Family: IL-7/ IL-9

Synonyms: IL7, IL-7, Interleukin-7, Interleukin 7

Gene ID: 3574

UniProt: P13232

Pathways: JAK-STAT Signaling