PF4 antibody

Catalog No. ABIN465152

The Rabbit Polyclonal anti-PF4 antibody is suitable to detect PF4 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for PF4 antibody (ABIN465152)

Target: PF4 (Platelet Factor 4 (PF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PF4 Antibody

Specificity: Human PF-4

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human PF-4.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1 mg/mL-2 mg/mL with an overnight incubation at 4 °C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hPF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PF-4 (LS-C104446) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: CXCL4 / PF4

Background: Name/Gene ID: PF4
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: PF4, C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4, Platelet factor 4

Gene ID: 5196

UniProt: P02776