PF4 antibody (AA 30-105)

Catalog No. ABIN4886693

Product Details anti-PF4 Antibody

Target: PF4 (Platelet Factor 4 (PF4))

Binding Specificity: AA 30-105

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Platelet factor 4(PF4) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Platelet factor 4(PF4) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Gene Name: platelet factor 4
Protein Name: Platelet factor 4

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse PF4 recombinant protein (Position: V30-S105). Mouse PF4 shares 74.3% and 86.8% amino acid (aa) sequence identity with human and rat PF4, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: PF4 (PF4 Products)

Synonyms: CXCL4 antibody, PF-4 antibody, SCYB4 antibody, Cxcl4 antibody, Scyb4 antibody, Pf4a antibody, RATPF4A antibody, PF4A antibody, CXCL4L1 antibody, CXCL4V1 antibody, PF4-ALT antibody, SCYB4V1 antibody, PF4 antibody, platelet factor 4 antibody, platelet factor 4 variant 1 antibody, PF4 antibody, Pf4 antibody, LOC703451 antibody, PF4V1 antibody, LOC100524373 antibody, LOC102181854 antibody, LOC100630489 antibody

Background: Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Synonyms: Platelet factor 4(PF4) | Platelet factor 4(PF-4) | platelet factor4 | CXCL4 | Iroplact | OncostatinA | Oncostatin-A | PF4 | PF-4 | PLF4 | SCYB 4 | SCYB4 | P02776

Gene ID: 56744

UniProt: Q9Z126