VEGFA antibody

Catalog No. ABIN465162

The Mouse Monoclonal anti-VEGFA antibody (ABIN465162) specifically detects VEGFA in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for VEGFA antibody (ABIN465162)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-VEGFA Antibody

Specificity: Human VEGF

Purification: Protein A purified

Immunogen: E.coli derived recombinant Human VEGF165.

Type of Immunogen: Cell extract

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 4 μg/mL), WB (0.25 - 0.5 μg/mL)

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10 ng/mL), a concentration of &le. 0.2 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with biotinylated antigen affinity purified anti-human VEGF165 (LS-C104615) as the detection antibody at a concentration of approximately 0.25-1 μg/mL. Western Blot: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA / VEGF

Background: Name/Gene ID: VEGFA
Family: PDGF

Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1

Gene ID: 7422

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling