CCL7 antibody

Catalog No. ABIN465166

This Mouse Monoclonal antibody specifically detects CCL7 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for CCL7 antibody (ABIN465166)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL7 Antibody

Specificity: Human MCP-3

Purification: Protein A purified

Immunogen: E.coli derived recombinant Human MCP-3.

Type of Immunogen: Cell extract

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 4 μg/mL), Neut (1 - 3 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human MCP-3 (100 ng/mL), a concentration of 1-3 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect recombinant human MCP-3 when used with biotinylated antigen affinity purified anti-human MCP-3 (LS-C104664) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2-4 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: CCL7 / MCP-3

Background: Name/Gene ID: CCL7
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL7, C-C motif chemokine 7, Chemokine (C-C motif) ligand 7, FIC, MARC, MCP3, Monocyte chemotactic protein 3, SCYA7, Small-inducible cytokine A7, MCP-3, SCYA6, NC28

Gene ID: 6354

UniProt: P80098