WISP2 antibody

Catalog No. ABIN465385

This Rabbit Polyclonal antibody specifically detects WISP2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for WISP2 antibody (ABIN465385)

Target: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WISP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-WISP2 Antibody

Specificity: Human CTGFL/WISP-2

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human CTGFL/WISP-2.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Additional protein blocks were used in conjunction with the secondary reagents. Optimal concentrations and conditions may vary. ELISA: To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGFL/WISP-2 (LS-C104595) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for WISP2

Target: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Alternative Name: WISP2

Background: Name/Gene ID: WISP2

Synonyms: WISP2, CCN5, CCN family member 5, CTGFL, CT58, CTGF-L, WISP-2

Gene ID: 8839

UniProt: O76076

Pathways: WNT Signaling, Growth Factor Binding