FGF1 antibody (Biotin)

Catalog No. ABIN465423

The Biotin-conjugated Rabbit Polyclonal anti-FGF1 antibody (ABIN465423) specifically detects FGF1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for FGF1 antibody (Biotin) (ABIN465423)

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-FGF1 Antibody (Biotin)

Specificity: Human FGF-acidic

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human FGF-acidic.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hFGF-acidic by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-acidic. To detect hFGF-acidic by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human FGF-acidic (LS-B8222) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-acidic. Western Blot: To detect hFGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-acidic is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for FGF1

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Alternative Name: FGF1

Background: Name/Gene ID: FGF1
Family: HBGF

Synonyms: FGF1, AFGF, Endothelial cell growth factor, Fibroblast growth factor 1, FGFA, ECGFB, HBGF-1, GLIO703, ECGF, ECGF-beta, ECGFA, FGF-1, FGF-alpha, HBGF1

Gene ID: 2246

UniProt: P05230

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway