CCL7 antibody

Catalog No. ABIN465455

The Goat Polyclonal anti-CCL7 antibody is suitable to detect CCL7 in samples from Mouse. It has been validated for WB, ELISA and Neut.

Quick Overview for CCL7 antibody (ABIN465455)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL7 Antibody

Specificity: Mouse MCP-3

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine MCP-3.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (4 - 6 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Murine MCP-3 (100 ng/mL), a concentration of 4-6 μg/mL of this antibody is required. ELISA: To detect mMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MCP-3 (LS-C104575) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMCP-3. Western Blot: To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: CCL7 / MCP-3

Background: Name/Gene ID: CCL7
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL7, C-C motif chemokine 7, Chemokine (C-C motif) ligand 7, FIC, MARC, MCP3, Monocyte chemotactic protein 3, SCYA7, Small-inducible cytokine A7, MCP-3, SCYA6, NC28

Gene ID: 6354

UniProt: P80098