CCL21 antibody (Biotin)

Catalog No. ABIN465485

This Rabbit Polyclonal antibody specifically detects CCL21 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CCL21 antibody (Biotin) (ABIN465485)

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL21 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CCL21 Antibody (Biotin)

Specificity: Human Exodus-2

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Exodus-2.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hExodus-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hExodus-2. To detect hExodus-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Exodus-2 (LS-B7413) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hExodus-2. Western Blot: To detect hExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hExodus-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL21

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Alternative Name: CCL21 / Exodus 2 / SLC

Background: Name/Gene ID: CCL21
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL21, 6Ckine, Beta chemokine exodus-2, C-C motif chemokine 21, Beta-chemokine exodus-2, CKb9, ECL, Small-inducible cytokine A21, SCYA21, SLC, Exodus-2, TCA4

Gene ID: 6366

UniProt: O00585

Pathways: Regulation of Actin Filament Polymerization