CCL8 antibody

Catalog No. ABIN465585

The Rabbit Polyclonal anti-CCL8 antibody (ABIN465585) specifically detects CCL8 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for CCL8 antibody (ABIN465585)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CCL8 Antibody

Specificity: Human MCP-2

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MCP-2.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal gastric mucosa. The recommended concentration is 0.75 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hMCP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MCP-2 (LS-C104722) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-2. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: CCL8 / MCP-2

Background: Name/Gene ID: CCL8
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL8, C-C motif chemokine 8, HC14, MCP2, Monocyte chemotactic protein 2, Scya10, SCYA8, Small-inducible cytokine A8, MCP-2, Chemokine (C-C motif) ligand 8

Gene ID: 6355

UniProt: P80075