CCL8 antibody (AA 24-97)

Catalog No. ABIN6719605

Product Details anti-CCL8 Antibody

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Binding Specificity: AA 24-97

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL8 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for CCL8 detection. Tested with IHC-P, ELISA(Cap) in Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for CCL8 detection. Tested with IHC-P, ELISA(Cap) in Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse CCL8 recombinant protein (Position: G24-P97).

Isotype: IgG

Application Details

Application Notes:

Application details: Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL ELISA(Cap)|1-5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: Ccl8 (CCL8 Products)

Synonyms: HC14 antibody, MCP-2 antibody, MCP2 antibody, SCYA10 antibody, SCYA8 antibody, 1810063B20Rik antibody, AB023418 antibody, Mcp2 antibody, Scya8 antibody, C-C motif chemokine ligand 8 antibody, chemokine (C-C motif) ligand 8 antibody, C-C motif chemokine 8 antibody, monocyte chemoattractant protein-2 precursor antibody, CCL8 antibody, Ccl8 antibody, LOC101119572 antibody, MCP-2 antibody

Background:

Synonyms: C-C motif chemokine 8, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, MCP-2, Small-inducible cytokine A8, Ccl8, Mcp2, Scya8

Background: CCL8, also known as monocyte chemoattractant protein 2 (MCP2), is a protein that in humans is encoded by the CCL8 gene. It is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. MCP-2 is chemotactic for and activates many different immune cells, including mast cells, eosinophils and basophils, (that are implicated in allergic responses), and monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B, CCR3 and CCR5.

Gene ID: 20307

UniProt: Q9Z121