Thymic Stromal Lymphopoietin antibody

Catalog No. ABIN465621

The Rabbit Polyclonal anti-Thymic Stromal Lymphopoietin antibody is suitable to detect Thymic Stromal Lymphopoietin in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Thymic Stromal Lymphopoietin antibody (ABIN465621)

Target: Thymic Stromal Lymphopoietin (TSLP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thymic Stromal Lymphopoietin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Thymic Stromal Lymphopoietin Antibody

Specificity: Human TSLP

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TSLP.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human prostate. The recommended concentration is 0.125 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hTSLP by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TSLP (LS-C104690) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTSLP. Western Blot: To detect hTSLP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTSLP is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background: Name/Gene ID: TSLP
Family: Cytokine

Synonyms: TSLP, Thymic stromal lymphopoietin

Gene ID: 85480

UniProt: Q969D9