IL-21 antibody

Catalog No. ABIN465670

This Rabbit Polyclonal antibody specifically detects IL-21 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for IL-21 antibody (ABIN465670)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IL-21 Antibody

Specificity: Mouse IL-21

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine IL-21.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect mIL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-21 (LS-C104548) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-21. Western Blot: To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-21 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL deionized water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-21

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL21

Background: Name/Gene ID: IL21
Family: Interleukin

Synonyms: IL21, Interleukin 21, Interleukin-21, Interleukin-21 isoform, Za11, IL-21

Gene ID: 59067

UniProt: Q9HBE4

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response