CXCL9 antibody

Catalog No. ABIN465878

This Rabbit Polyclonal antibody specifically detects CXCL9 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for CXCL9 antibody (ABIN465878)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL9 Antibody

Specificity: Human MIG

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIG.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (5 - 7 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.25 μg/mL-0.50 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a  pH 6 sodium citrate buffer. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hMIG (100 ng/mL), a concentration of 5-7 μg/mL of this antibody is required. ELISA: To detect hMIG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIG (LS-C104825) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIG. Western Blot: To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Alternative Name: MIG / CXCL9

Background: Name/Gene ID: CXCL9
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL9, Crg-10, Humig, SCYB9, Small-inducible cytokine B9, C-X-C motif chemokine 9, CMK, MIG

Gene ID: 4283

UniProt: Q07325