Acetylated Lysine antibody

Catalog No. ABIN479094

The Rabbit Polyclonal anti-Acetylated Lysine antibody (ABIN479094) specifically detects Acetylated Lysine in WB, ELISA, IHC, IP and IHC (p).

Quick Overview for Acetylated Lysine antibody (ABIN479094)

Target: Acetylated Lysine

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetylated Lysine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Acetylated Lysine Antibody

Specificity: Detects proteins posttranslationally modified by acetylation on the epsilon-amino groups of lysine residues. Recognizes acetylated lysine in a wide range of sequence contexts. It has been demonstrated to recognize acetylated histones, p53, CBP, PCAF and chemically acetylated BSA. Has been shown to react with as little as 0.04 ng of chemically acetylated BSA while not recognizing up to 25 μg of nonacetylated BSA.

Purification: Immunoaffinity purified

Immunogen: Synthetic acetylated- lysine peptide (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:1000), IHC, IHC-P, IP (1:100), WB (1:1000)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and ELISA. Western Blot: 1:1000. Immunoprecipitation: 1:100. Immunohistochemistry: 1:50. ELISA: 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Acetylated Lysine

Target: Acetylated Lysine

Alternative Name: Acetyl-Lysine

Target Type: Chemical