B-Cells antibody

Antigen: B-Cells

Clonality: Monoclonal (Ki-B1R)

Host: Mouse

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN481701

Additional Information

Alternative name: B cells

Immunogen: Lymphocytes

Clone: Ki-B1R

Description: Gene: B cells

Specificity: Ki-B1R recognizes a surface antigen of 47kDa molecular weight, which appears as a single band under reducing conditions in SDS-PAGE. The epitope has not been further characterized. Antigen distribution: Isolated cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19% + 3% of peripheral blood lymphocytes (n=5). In the same test, 18% ± 3% of the lymphocytes stained positive for sIg, whereas 79% ± 3% stained positive with Ki-T1R, the rat pan T-cell marker. Tissue sections: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B- lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo. Specificity: Rat: mature B-lymphocytes, plasma cells. Other species: not tested. Predicted Reactivities: Rat (Rattus).

Application Details

Application Notes: IHC-P (1:100)

Purification: Affinity Purified

Buffer: Lyophilized from PBS pH 7.2, 10mg/ml BSA, 0.01% thimerosal, Resuspension: 0.5ml distilled water

Storage: Store at 4°C.

Restrictions: For Research Use only