PKC antibody (pThr497)

Catalog No. ABIN482120

The Rabbit Polyclonal anti-PKC antibody is suitable to detect PKC in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC.

Quick Overview for PKC antibody (pThr497) (ABIN482120)

Target: PKC (Protein Kinase C (PKC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-PKC Antibody

Binding Specificity: AA 623-672, pThr497

Specificity:

PKC-pan (Phospho-Thr497) Antibody detects endogenous levels of PKC-pan only when phosphorylated at Thr497.

PhosphorylationH:T497/500/507/566/514/512/538/410 M:T497/500/505/566/514/513/538/410 R:T497/500/505/566/514/513/538/410

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC-pan around the phosphorylation site of Thr497.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

Comment:

Unigene-Number: Hs.531704, Hs.708867, Hs.460355, Hs.155342, Hs.580351, Hs.631564, Hs.333907, Hs.498570, Hs.496255 (NCBI Gene Symbol: PRKCA)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PKC

Target: PKC (Protein Kinase C (PKC))

Alternative Name: PKC

Background:

Synonyms: KPCA, PKC III, PKC-A, PKC-III, PKC-alpha, PRKACA, PRKCA, Protein kinase C, alpha type, kinase PKC-alpha PKC-delta, PRKCD, kinase PKC-delta, nPKC-delta PKC-epsilon, PKCEA, PRKCE, epsilon type, kinase PKC-epsilon, nPKC-epsilon

NCBI Gene Symbol: PRKCA

Molecular Weight: 76 kDa

Gene ID: 5578, 5579, 5580

OMIM: 176960

UniProt: P17252, P05771, Q05655, Q02156, P05129, P24723, Q04759, Q05513

Pathways: TCR Signaling, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Phototransduction