BAK1 antibody (AA 1-50)

Catalog No. ABIN483832

This Rabbit Polyclonal antibody specifically detects BAK1 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse. It has been mentioned in 2+ publications

Quick Overview for BAK1 antibody (AA 1-50) (ABIN483832)

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-BAK1 Antibody

Binding Specificity: AA 1-50

Specificity:

Bak Antibody detects endogenous levels of total Bak protein.

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Bak.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.485139 (NCBI Gene Symbol: BAK1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-BAK1 antibody (ABIN483832)

Plötz, Gillissen, Hossini, Daniel, Eberle: "Disruption of the VDAC2-Bak interaction by Bcl-x(S) mediates efficient induction of apoptosis in melanoma cells." in: Cell death and differentiation, Vol. 19, Issue 12, pp. 1928-38, (2012) (PubMed).

Plötz, Hossini, Gillissen, Daniel, Stockfleth, Eberle: "Mutual regulation of Bcl-2 proteins independent of the BH3 domain as shown by the BH3-lacking protein Bcl-x(AK)." in: PLoS ONE, Vol. 7, Issue 4, pp. e34549, (2012) (PubMed).

Target Details for BAK1

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Alternative Name: Bak

Background:

Synonyms: Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like 7 protein, BAK1, BAK, BCL2L7

NCBI Gene Symbol: BAK1

Molecular Weight: 23 kDa

Gene ID: 578

OMIM: 600516

UniProt: Q16611, Q13014

Pathways: Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response