ZNF496 antibody (Middle Region)

Catalog No. ABIN486781

This Rabbit Polyclonal antibody specifically detects ZNF496 in WB. It exhibits reactivity toward Mouse, Human, Rat, Cow, Sheep, Dog, Horse, Pig and Rabbit.

Quick Overview for ZNF496 antibody (Middle Region) (ABIN486781)

Target: ZNF496 (Zinc Finger Protein 496 (ZNF496))

Reactivity: Mouse, Human, Rat, Cow, Sheep, Dog, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF496 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ZNF496 Antibody

Binding Specificity: Middle Region

Sequence: QEKPHECSVC GELFSDSEDL DGHLESHEAQ KPYRCGACGK SFRLNSHLLS

Predicted Reactivity: Cow: 86%, Dog: 93%, Horse: 86%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 93%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against ZNF496. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ZNF496

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 587 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ZNF496

Target: ZNF496 (Zinc Finger Protein 496 (ZNF496))

Alternative Name: ZNF496

Background: ZNF496 contains a SCAN box, a KRAB-A domain, and four consensus C2H2-type zinc fingers preceded by a unique finger derivative, referred to herein as the C2HR motif. The C2HR motif functions to mediate protein-protein interaction with the cysteine-rich (C5HCH) domain of NSD1 in a Zn(II)-dependent fashion, and when tethered to RNA polymerase II promoters, represses transcription in an NSD1-dependent manner. ZNF496 contains a novel type of zinc finger motif that functions as a docking site for NSD1 and is more than just a degenerate evolutionary remnant of a C2H2 motif.
Alias Symbols: MGC15548, NIZP1, ZKSCAN17, ZFP496
Protein Interaction Partner: ZKSCAN4, ZSCAN22, ZNF483, ZNF446, ZSCAN12, ZSCAN21, ARR3, Nsd1, SUMO2, UBC, CBX3, TRIM28, NR3C1, POLR3D, ZZZ3, JADE2, RAI1, SSX3, SUPT5H, PPARG,
Protein Size: 587

Molecular Weight: 67 kDa

Gene ID: 84838

NCBI Accession: NM_032752, NP_116141

UniProt: Q96IT1