Spt6 antibody (N-Term)

Catalog No. ABIN487062

The Rabbit Polyclonal anti-Spt6 antibody (ABIN487062) specifically detects Spt6 in WB. The antibody is reactive with Mouse, Rat, Dog, Cow, Horse and Rabbit samples.

Quick Overview for Spt6 antibody (N-Term) (ABIN487062)

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Reactivity: Mouse, Rat, Dog, Cow, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Spt6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Spt6 Antibody

Binding Specificity: N-Term

Purpose: SUPT6H Antibody - N-terminal region

Sequence: EGSDSGDSED DVGHKKRKRT SFDDRLEDDD FDLIEENLGV KVKRGQKYRR

Predicted Reactivity: Cow: 85%, Dog: 100%, Horse: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SUPT6H. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SUPT6H

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33387-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Spt6

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Alternative Name: SUPT6H

Background:

Background Information: SUPT6H may be functionally analogous to SPT6 and emb-5 and may therefore regulate transcription through establishment or maintenance of chromatin structure. Spt6 may also participates in the regulation of transcription by RNA polymerase II (RNAPII). Human Spt6 (hSpt6) is a classic transcription elongation factor that enhances the rate of RNAPII elongation. HSpt6 is capable of stimulating transcription elongation both individually and in concert with DRB sensitivity-inducing factor (DSIF).

Gene Name: Suppressor of Ty 6 homolog (S. cerevisiae)

Alternative Symbols: SPT6, SPT6H, emb-5

Protein Name: Transcription elongation factor SPT6

Molecular Weight: 199kDa

Gene ID: 6830

NCBI Accession: NP_003161

UniProt: Q7KZ85

Pathways: Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response