Toc75 antibody (Chloroplastic, Domain 3)

Catalog No. ABIN5684021

The Rabbit Polyclonal anti-Toc75 antibody is suitable to detect Toc75 in samples from Pea, Synechococcus sp., Synechocystis, Thermosynechococcus, Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomytrium Patens, Spinach and Thellungiella salsuginea. It has been validated for FACS, IL and WB.

Quick Overview for Toc75 antibody (Chloroplastic, Domain 3) (ABIN5684021)

Target: Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))

Reactivity: Pea, Synechococcus sp., Synechocystis, Thermosynechococcus, Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomytrium Patens, Spinach, Thellungiella salsuginea

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Toc75 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunolocalization (IL), Western Blotting (WB)

Product Details anti-Toc75 Antibody

Binding Specificity: Chloroplastic, Domain 3

Purpose: Anti-Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3

Cross-Reactivity (Details): Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Aegilops tauschii,Ananas comosus,Anthurium amnicola,Capsicum annuum,Catalpa bungei,Cicer arietinum,Cucumis melo,Glycine soja,Gossypium arboreum,Medicago truncatula,Morus notabilis,Nelumbo nucifera,Nicotiana sylvestris,Noccaea caerulescens,Populus trichocarpa,Ricinus communis,Sorghum bicolor,Theobroma cacao,Zea mays,Zostera marina,Vigna radiata,Vitis vinifera

Purification: Serum

Immunogen: Pisum sativum TOC75, Predicted POTRA Domain #3, Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. UniProt: Q43715

Application Details

Application Notes: 1 : 500 (Flow cyt), 1 : 100 (IL), 1 : 2000-1 : 100 000 (WB)

Comment:

antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for Toc75

Target: Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))

Background: OEP 75 or Toc75, Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446), In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87, Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane, YaeT, outer membrane protein assembly complex, (TIGR03303), FhaC, Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]

Molecular Weight: 88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

UniProt: Q43715