Toc75 antibody
Toc75
Reactivity: Pisum sativum, Synechococcus sp., Thermosynechococcus, Synechocystis
FACS, IL, WB
Host: Rabbit
Polyclonal
unconjugated
1 image
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- Target See all Toc75 products
- Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))
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Reactivity
- Pisum sativum, Synechococcus sp., Thermosynechococcus, Synechocystis
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Toc75 antibody is un-conjugated
- Application
- Flow Cytometry (FACS), Immunolocalization (IL), Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- Ricinus communis, Vitis vinifera, Populus trichocarpa
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 88 / 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
- Purification
- serum
- Immunogen
- psTOC75, Predicted POTRA Domain #1, Amino acids, 158-241, Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715
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anti-Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75) (Chloroplastic), (Domain 3) antibody
Toc75 Reactivity: Pisum sativum, Synechococcus sp., Thermosynechococcus, Nicotiana tabacum, Spinach, Arabidopsis thaliana FACS, IL, WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- 1: 2000 - 1: 25 000, 1: 100 000 (WB), 1: 500 (FACS). 1: 100 (IL)
- Comment
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antibody detects Toc75 POTRA domain 1 as purified protein, in chloroplast fraction and in crude envelope fraction
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 μL of sterile water
- Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))
- Abstract
- Toc75 Products
- Background
- OEP 75 or Toc75, Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446), In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87, Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane, YaeT, outer membrane protein assembly complex, (TIGR03303), FhaC, Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
- Molecular Weight
- expected: 88 kDa, apparent: 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
- Gene ID
- 158241
- UniProt
- Q43715
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