HSP70/HSC70 antibody (C-Term)

Catalog No. ABIN488588

There is 1 publication for this product available. The Rabbit Polyclonal anti-HSP70/HSC70 antibody is suitable to detect HSP70/HSC70 in samples from Chicken, Cow, Rat, Pig, Frog, C. elegans, Seal, Arabidopsis thaliana, Salmon, Rainbow Trout, Mummichog, Acanthamoeba castellanii and Dictyostelium sp.. It has been validated for WB.

Quick Overview for HSP70/HSC70 antibody (C-Term) (ABIN488588)

Target: HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))

Reactivity: Chicken, Cow, Rat, Pig, Frog, C. elegans, Seal, Arabidopsis thaliana, Salmon, Rainbow Trout, Mummichog, Acanthamoeba castellanii, Dictyostelium sp.

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP70/HSC70 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSP70/HSC70 Antibody

Binding Specificity: C-Term

Purpose: Anti-HSP70/HSC70 | Heat shock protein 70

Cross-Reactivity (Details): Arabidopsis thaliana, Acanthamoeba castellanii (amoeba), Caenorhabditis elegans, salmon (Salmo salar), Dictyostelium discoideum, frog-heart, Frog-skeletal muscle, Frog-liver, rainbow trout (Oncorhynchus mykiss), cow, Chicken, pig, Rat, seal, mummichog
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Gammarus pulex,Salmo salar (Atlantic salmon),Salvelinus fontinalis (Brook trout)

Purification: Serum

Immunogen: KLH-conjugated C-terminal synthetic peptide conserved in hsc/hsp70 sequences from a wide range of animal species

Application Details

Application Notes: 1 : 10 000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-HSP70/HSC70 antibody (ABIN488588)

Chandra, Bosker, Hogan, Lister, MacLatchy, Currie: "Sustained high temperature increases the vitellogenin response to 17α-ethynylestradiol in mummichog (Fundulus heteroclitus)." in: Aquatic toxicology (Amsterdam, Netherlands), Vol. 118-119, pp. 130-40, (2012) (PubMed).

Target Details for HSP70/HSC70

Target: HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))

Alternative Name: HSP70/HSC70

Background:

Background: Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. A killifish is a large family of various oviparous (egg-laying) cyprinodontiform fish including 1270 different species.

Additional information: Chosen peptide sequence is also conserved in several fish species including: DQ202278.1 HSC70 Fundulus, DQ202279.1 HSP70-1 Fundulus, DQ202280.1 HSP70-2 Fundulus, BT059361.1 HSC70 Salmo salar (atlantic salmon), AB092839.2 HSP70 Carassius auratus (goldfish), BC056709.1 HSP70 Danio rerio (zebrafish) and other animal HSP70 proteins

Molecular Weight: 70 kDa