Phosphotyrosine antibody

Catalog No. ABIN488674

This Mouse Monoclonal antibody specifically detects Phosphotyrosine in WB, IP, IF and IHC. It has been mentioned in 1 publication

Quick Overview for Phosphotyrosine antibody (ABIN488674)

Target: Phosphotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: G104

Product Details anti-Phosphotyrosine Antibody

Specificity: Antibody reacts with phosphotyrosine and detects the presence ofphosphotyrosine in proteins of both unstimulated and stimulated cell lysated.

Cross-Reactivity (Details): Does not cross react with phosphoserine or phosphothreonine

Predicted Reactivity: antibody is not species specific

Purification: affinity purified

Immunogen: Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3-™-dimentrylaminopropyl) carbodiimide

Isotype: IgG1

Application Details

Application Notes: 1 : 1000 with standard ECL (WB)

Comment:

1 μg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 μg of rat tissue lysate by colorimetric immunoblot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: protein G purified IgG1 in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of thetubes.

Storage: -20 °C

Storage Comment: store at -20°C for one year, make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Expiry Date: 12 months

References for anti-Phosphotyrosine antibody (ABIN488674)

Garton, Tonks: "Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST." in: The Journal of biological chemistry, Vol. 274, Issue 6, pp. 3811-8, (1999) (PubMed).

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.