Acetylated Lysine antibody

Antigen: Acetylated Lysine

Clonality: Monoclonal (7F8)

Host: Mouse

Reactivity: Cow (Bovine), Bird (Avian)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN488678

Additional Information

Immunogen: acetylated KLH

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 7F8

Description: Post-translational modifications of proteins play critical roles in the regulation andfunction of many known biological processes. Proteins can be post-translationallymodified in many different ways, and a common posttranscriptional modification ofLysine involves acetylation (1). The conserved amino-terminal domains of the fourcore histones (H2A, H2B, H3 and H4) contain lysines that are acetylated byhistone acetyltransferases (HATs) and deacetylated by histone deacetylases(HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation anddeacetylation have been recognized as an emerging intracellular signalingmechanism that plays critical roles in regulating gene transcription, cell-cycleprogression, apoptosis, DNA repair, and cytoskeletal organization (3).

Specificity: Predicted reactivity: higher plants.

Application Details

Application Notes: Recommended Dilution needs to be determined for each application.

Buffer: protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 %glycerol at concentration 1 mg/ml antibody detects proteins containing acetylated lysine residues in ELISA andWBs. Does not detect non-acetylated lysine residues.

Storage: store at -20°C, avoid repeated freeze-thaw cycles. Please, remember to spintubes briefly prior to opening them to avoid any losses that might occur fromlyophilized material adhering to the cap or sides of the tubes.

Restrictions: For Research Use only