LIME antibody (AA 86-202)

Catalog No. ABIN490334

The Mouse Monoclonal anti-LIME antibody is suitable to detect LIME in samples from Mouse. It has been validated for WB and IP.

Quick Overview for LIME antibody (AA 86-202) (ABIN490334)

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIME antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: MLIME-05

Product Details anti-LIME Antibody

Binding Specificity: AA 86-202

Specificity: Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kD Lck-interacting transmembrane adaptor expressed mainly by T cells.

Purification: Protein A purified

Immunogen: Recombinant protein corresponding to amino acids 86-202 of mouse LIME.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: IP, WB

Usage: Immunoprecipitation: Positive control - murine splenic T cells. Western Blotting: Application note - reducing conditions. Recommended dilution - 1-5 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Alternative Name: LIME1 / LIME

Background: Name/Gene ID: LIME1

Synonyms: LIME1, DJ583P15.4, Lck-interacting molecule, LIME, RP4-583P15.5, LP8067

Gene ID: 54923

UniProt: Q9H400