CAMK2A antibody (pThr286)

Catalog No. ABIN492420

The Mouse Monoclonal anti-CAMK2A antibody (Clone 22B1) (ABIN492420) specifically detects CAMK2A in WB, IF, IP, EIA and IHC (fro). The antibody is reactive with Human and Rat samples.

Quick Overview for CAMK2A antibody (pThr286) (ABIN492420)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 22B1

Product Details anti-CAMK2A Antibody

Binding Specificity: pThr286

Cross-Reactivity (Details): Species reactivity (tested):Rat, Human.

Characteristics: Synonyms: CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-IIsubunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha

Purification: Protein G Chromatography.

Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunflourescence: 10 μg/mL. Immunoprecipitaion. ImmunohistochemistryWestern blot: 1 μg/mL, A band of approx. 50 kDa is detected.1 μg/mL of CAMKII was sufficient for detection of 0.2 μg CAMKII by colorimetric immunoblot. (Ref.13)

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4 containing 0.09 % Sodium Azide in 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CAMK2A

Background: CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca 2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha, KIAA0968

Gene ID: 25400

UniProt: P11275

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction