XBP1 antibody (AA 2-160)

Catalog No. ABIN492538

This Mouse Monoclonal antibody specifically detects XBP1 in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for XBP1 antibody (AA 2-160) (ABIN492538)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 9B7E5

Product Details anti-XBP1 Antibody

Binding Specificity: AA 2-160

Specificity: Recognizes XBP1/TREB5 (2-160).

Characteristics: Synonyms: XBP-1, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5

Purification: Immunoaffinity Chromotography.

Immunogen: XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to160 of Human XBP-1 (Genbank accession No. BAB82982).

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: XBP1 antibody can be used for detection of XBP1 at 0.5-1 μg/mL. Positive Control: XBP-1 Recombinant Protein. Immunocytochemistry: 2 μg/mL. Positive Control: HepG2 cells. Immunohistochemistry on Paraffin Sections: 10 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1 / TREB5

Background: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) (1). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94 (3,4).Synonyms: Tax-responsive element-binding protein 5, X-box-binding protein 1, XBP-1, XBP2

Gene ID: 7494

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response