LC3B antibody (Internal Region)

Catalog No. ABIN492616

This Mouse Monoclonal antibody specifically detects LC3B in WB and IF. It exhibits reactivity toward Human.

Quick Overview for LC3B antibody (Internal Region) (ABIN492616)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 5H3

Product Details anti-LC3B Antibody

Binding Specificity: Internal Region

Specificity: Recognizes LC3A, LC3B and LC3C

Characteristics: Synonyms: LC3A, LC3B, LC3C, Microtubule-associated protein 1 light chain 3, Autophagy-relatedprotein LC3, Autophagy-related ubiquitin-like modifier LC3

Purification: Purified from Serum-Free Cell Culture Supernatant by Subsequent Ultrafiltration and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to hemocyaninEpitope: Internal sequence that is identical in LC3A, LC3B and LC3C

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 μg/mL for HRPO/ECL detectionRecommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. We strongly recommend to use PVDF membranes for immunoblot analysis. Immunocytochemistry: Use at 1-10 μg/mL (Paraformaldehyd/Methanol fixation). Included Positive Control: Cell lysate from untreated Neuro 2A (See Protocols).

Protocol: Positive Control: Cell lysate from untreated Neuro 2A cells, brain endothelioma (Mouse)Format: Lyophilized cell lysate from serum starved Neuro 2A. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS with 0.09 % Sodium Azide, PEG, Sucrose and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: LC3B

Background: Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (microtubule-associated protein1 light chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.Synonyms: Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A / 1B light chain 3 B, MAP1A/MAP1B, MAP1LC3B, Map1alc3, Map1lc3, Map1lc3b, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B

Gene ID: 81631

UniProt: Q9GZQ8

Pathways: Autophagy