Mouse anti-Human IgE (Chain epsilon) Antibody

Catalog No. ABIN492642

Quick Overview for Mouse anti-Human IgE (Chain epsilon) Antibody (ABIN492642)

Target: IgE

Clonality: Monoclonal

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Clone: B3102E8

Isotype: IgG1

Product Details

Binding Specificity: Chain epsilon

Reactivity: Human

Host: Mouse

Purification: Purified

Application Details

Application Notes: ELISA (Recommended as a Detection Antibody). Western blotting. Dot- and slot-immunoblotting. Immunocytochemistry. Immunohistochemistry on Frozen Sections.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate buffered saline, pH 8.2 without preservatives or amine-containing buffer salts.

Preservative: Without preservative

Handling Advice: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C

Target Details

Target: IgE

Target Type: Antibody

Background: IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/mL, compared to 10 mg/mL for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.Synonyms: Immunoglobulin E human