HNRNPC antibody (N-Term)

Catalog No. ABIN497942

The Rabbit Polyclonal anti-HNRNPC antibody is suitable to detect HNRNPC in samples from Human, Rat, Mouse, Rabbit and Cow. It has been validated for WB, IF and IHC (p).

Quick Overview for HNRNPC antibody (N-Term) (ABIN497942)

Target: HNRNPC (Heterogeneous Nuclear Ribonucleoprotein C (C1/C2) (HNRNPC))

Reactivity: Human, Rat, Mouse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HNRNPC Antibody

Binding Specificity: AA 1-142, N-Term

Specificity: Recognizes hnRNP-C1/C2/HNRNPC (1-152).
Cellular Localization: Nucleus.

Cross-Reactivity (Details): Species reactivity (expected):Mouse (100 %), Rat (100 %), Bovine (100 %) and Rabbit (92 %)Species reactivity (tested):Human.

Purification: Antigen-Affinity Chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 142 of hnRNP-C1/C2.

Application Details

Application Notes: Western blotting: 1/500-1/3000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/100-1/250. Positive Controls: 293T, A431, HeLa, HepG2, Molt-4, Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.79 mg/mL

Buffer: 0.1 M Tris, 0.1 M Glycine, 20 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for HNRNPC

Target: HNRNPC (Heterogeneous Nuclear Ribonucleoprotein C (C1/C2) (HNRNPC))

Alternative Name: HnRNP-C1/C2 / HNRNPC

Background: HnRNP-C1/C2 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene.Synonyms: HNRPC, Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1 / hnRNP C2

Gene ID: 3183, 9606

UniProt: P07910