LAMP1 antibody

Catalog No. ABIN497981

The Rabbit Polyclonal anti-LAMP1 antibody has been validated for WB, IF and EIA. It is suitable to detect LAMP1 in samples from Human, Mouse and Rat.

Quick Overview for LAMP1 antibody (ABIN497981)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-LAMP1 Antibody

Specificity: Recognizes CD107a/LAMP1 (Center).

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat

Purification: Immunoaffinity Chromatography

Immunogen: LAMP1 antibody was raised against a 15 amino acid peptide from near the center of Human LAMP-1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: CD107a/LAMP1 antibody can be used for the detection of CD107a/LAMP1 at1-2 μg/mL. Despite its predicted size, LAMP-1 migrates at 110 kDa in SDS-PAGE. Positive Control: EL4 Cell Lysate. Immunofluorescence. Positive Control: Human Colon Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Alternative Name: CD107a / LAMP1

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome (4,5). LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression (6). However, the loss of both proteins resulted in embryonic lethality (7), suggesting that each protein possesses some unique and necessary functions.Synonyms: CD107 Antigen-like Family Member A, LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1

Gene ID: 3916

NCBI Accession: NP_005552

UniProt: P11279

Pathways: Autophagy