IGFBP7 antibody (Internal Region)

Catalog No. ABIN498022

This Goat Polyclonal antibody specifically detects IGFBP7 in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IGFBP7 antibody (Internal Region) (ABIN498022)

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IGFBP7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IGFBP7 Antibody

Binding Specificity: AA 145-159, Internal Region

Sequence: EKAITQVSKG TCEQG

Specificity: Recognizes IGFBP7 (145-159).

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_006423.1. Genename: IGFBP7

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 1-3 μg/mL. Approx 30 kDa band observed in Mouse Kidney lysates (calculatedMW of 29.0 kDa according to Human NP_001544.1 and to Mouse NP_032074.3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IGFBP7

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Alternative Name: IGFBP7

Background: IGF-BPs form high affinity complexes with both IGF-I and IGF-II and act to control of the distribution, function and activity of IGFs in various cell tissues and body fluids. There are seven named IGF-BPs. IGFBP7 plays a role in skeletal myogenesis by binding to IGF in a manner that inhibits IGF induced differentiation of skeletal myoblasts, without affecting IGF induced proliferation. Additionally, IGFBP7 suppresses growth and colony formation of prostate and breast cancer cell lines through an IGF independent mechanism, which causes a delay in the G1 phase of the cell cycle, and increased apoptosis. IGFBP7 is expressed in a wide range of normal human tissues and it usually shows reduced expression in cancer cell lines of prostate, breast, colon, and lung origin.Synonyms: IBP-7, IBP7, IGF-binding protein 7, IGFBP-7, IGFBP-rP1, Insulin-like growth factor-binding protein 7, MAC25, PGI2-stimulating factor, PSF, Prostacyclin-stimulating factor, TAF, Tumor-derived adhesion factor

Gene ID: 3490

NCBI Accession: NP_001544

UniProt: Q16270

Pathways: Growth Factor Binding