ACPP antibody (Internal Region)

Catalog No. ABIN498060

This anti-ACPP antibody is a Goat Polyclonal antibody detecting ACPP in WB, IHC (p) and EIA. Suitable for Human, Mouse, Rat, Cow and Dog.

Quick Overview for ACPP antibody (Internal Region) (ABIN498060)

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ACPP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ACPP Antibody

Binding Specificity: Internal Region

Sequence: YGIHKQKEKS RLQ

Specificity: This antibody is expected to recognize both isoforms (NP_001090.2, NP_001127666.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of Human ACPP according to NP_001090.2. Genename: ACPP

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.3-1 μg/mL. Detects a band of Approx 50 kDa in Human Prostate lysates. The observed molecular weightcorresponds to earlier findings in literature with different antibodies (Patel et al. , ClinChem.1986 Oct,32(10): 1832-5. PMID: 3530530).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Alternative Name: ACPP / Prostatic Acid Phosphatase

Background: Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase.Synonyms: ACP3, PAP, PSAP, Prostate acid phosphatase

Molecular Weight: 44.6kDa (NP_001090.2)

Gene ID: 55

NCBI Accession: NP_001090

UniProt: P15309

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process