ACAT1 antibody (Internal Region)

Catalog No. ABIN498064

This Goat Polyclonal antibody specifically detects ACAT1 in WB, IF and EIA. It exhibits reactivity toward Human, Mouse, Rat and Cow.

Quick Overview for ACAT1 antibody (Internal Region) (ABIN498064)

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ACAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-ACAT1 Antibody

Binding Specificity: AA 257-269, Internal Region

Sequence: C-KRVDFSKVPK LKT

Specificity: Recognizes Acetoacetyl Coenzyme A Thiolase/ACAT1 (257-269).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of Human ACAT1 according to NP_000010.1. Genename: ACAT1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.01-0.03 μg/mL. Detects a band of Approx 45 kDa in Human Liver lysates (calculated MW of 45.5 kDaaccording to NP_000010.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Alternative Name: ACAT1 / MAT

Background: ACAT1, acetoacetyl CoA thiolase, plays a major role in ketone body metabolism. It is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.Synonyms: Acetoacetyl-CoA thiolase T2, Acetyl-CoA acetyltransferase mitochondrial

Gene ID: 38

NCBI Accession: NP_000010

UniProt: P24752