ETFDH antibody (Internal Region)

Catalog No. ABIN498086

The Goat Polyclonal anti-ETFDH antibody is suitable to detect ETFDH in samples from Human, Mouse, Rat, Cow, Dog and Rabbit. It has been validated for WB and EIA.

Quick Overview for ETFDH antibody (Internal Region) (ABIN498086)

Target: ETFDH (Electron-Transferring-Flavoprotein Dehydrogenase (ETFDH))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This ETFDH antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ETFDH Antibody

Binding Specificity: Internal Region

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine and Rabbit.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EHDQPAHLTLRD, from the internal region of the protein sequence according to NP_004444.2. Genename: ETFDH

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/2000. Western blot: 1-3 μg/mL. Detects a band of Approx 70 kDa in Human, Mouse and Rat Heart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat, Canine (Dog), Bovine and Rabbit. Add. Information: Calculated Molecular Weight: 68.0 kDa according to NP_004444.2

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ETFDH

Target: ETFDH (Electron-Transferring-Flavoprotein Dehydrogenase (ETFDH))

Alternative Name: ETFDH

Background: ETFDH or Electron-transferring-flavoprotein dehydrogenase in the inner mitochondrial membrane accepts electrons from electron-transfer flavoprotein which is located in the mitochondrial matrix and reduces ubiquinone in the mitochondrial membrane. The protein is synthesized as a 67- kDa precursor which is targeted to mitochondria and processed in a single step to a 64- kDa mature form located in the mitochondrial membrane. Deficiency in electron-transferring-flavoprotein dehydrogenase have been demonstrated in some patients with type II glutaricacidemia. ETFDH accepts electrons from ETF and reduces ubiquinone.Synonyms: ETF-QO, ETF-ubiquinone oxidoreductase, Electron transfer flavoprotein-ubiquinone oxidoreductase

Gene ID: 2110

NCBI Accession: NP_004444

UniProt: Q16134

Pathways: Monocarboxylic Acid Catabolic Process