NDUFS2 antibody (Internal Region)

Catalog No. ABIN498088

This Goat Polyclonal antibody specifically detects NDUFS2 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Pig and Rabbit.

Quick Overview for NDUFS2 antibody (Internal Region) (ABIN498088)

Target: NDUFS2 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2, 49kDa (NADH-Coenzyme Q Reductase) (NDUFS2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This NDUFS2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NDUFS2 Antibody

Binding Specificity: Internal Region

Sequence: C-SRGVRQWQPD VE

Specificity: This antibody is expected to recognize both reported isoforms (NP_004541.1, NP_001159631.1).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Bovine, Porcine and Rabbit.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_004541.1, NP_001159631.1. Genename: NDUFS2

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/16000. Western blot: 0.1-0.3 μg/mL. Detects a band of Approx 48 kDa in Human, Mouse and Rat Heart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NDUFS2

Target: NDUFS2 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2, 49kDa (NADH-Coenzyme Q Reductase) (NDUFS2))

Alternative Name: NDUFS2

Background: NDUFS2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria.Synonyms: CI-49kD, Complex I-49kD, NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial

Molecular Weight: 51.9kDa according to NP_001159631.1)

Gene ID: 4720

NCBI Accession: NP_004541

UniProt: O75306