Ephrin B1 antibody

Catalog No. ABIN498124

This Rabbit Polyclonal antibody specifically detects Ephrin B1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Ephrin B1 antibody (ABIN498124)

Target: Ephrin B1 (EFNB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Ephrin B1 Antibody

Specificity: This antibody detects endogenous levels of Ephrin-B1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for Ephrin B1

Target: Ephrin B1 (EFNB1)

Alternative Name: Ephrin-B1

Background: Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family. Both ephrins and Eph receptors are broadly expressed through the ectoderm, mesoderm, and endoderm of vertebrate embryos. Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors, Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors. Ephrin B1 may play a role in constraining the orientation of longitudinally projecting axons.Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2

Molecular Weight: approx. 38 kDa

Gene ID: 1947

NCBI Accession: NP_004420

UniProt: P98172

Pathways: RTK Signaling