HAT1 antibody

Catalog No. ABIN498132

The Rabbit Polyclonal anti-HAT1 antibody (ABIN498132) specifically detects HAT1 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HAT1 antibody (ABIN498132)

Target: HAT1 (Histone Acetyltransferase 1 (HAT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HAT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HAT1 Antibody

Specificity: This antibody detects endogenous levels of HAT1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for HAT1

Target: HAT1 (Histone Acetyltransferase 1 (HAT1))

Alternative Name: HAT1

Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.Synonyms: Histone acetyltransferase type B catalytic subunit

Molecular Weight: approx. 49 kDa

Gene ID: 8520

NCBI Accession: NP_003633

UniProt: O14929