HAT1 antibody

Antigen: HAT1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN498132

Additional Information

Characteristics: Synonyms: Histone acetyltransferase type B catalytic subunit

Gene ID: 8520

Swiss-Prot: O14929

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: In the intact cell, DNA closely associates with histones and other nuclear proteins to formchromatin. The remodeling of chromatin is believed to be a critical component oftranscriptional regulation and a major source of this remodeling is brought about by theacetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminaltail domain of histone results in an allosteric change in the nucleosomal conformation andan increased accessibility to transcription factors by DNA. Conversely, the deacetylation ofhistones is associated with transcriptional silencing. Several mammalian proteins havebeen identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociatedfactor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (alsodesignated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified ashistone deacetylases.

Specificity: This antibody detects endogenous levels of HAT1 protein. Species Reactivity: Tested: Human, mouse, rat

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2

Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeatedfreezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only